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rabbit anti map2 polyclonal  (Proteintech)


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    Structured Review

    Proteintech rabbit anti map2 polyclonal
    Rabbit Anti Map2 Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+map2+antibody/MAP2+Antibody/pmc13045046-1-0-4
    Average 96 stars, based on 460 article reviews
    rabbit anti map2 polyclonal - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Defective biosynthesis of ascorbic acid in Sod1-deficient mice results in lethal damage to lung tissue.
    Article Snippet: Superoxide dismutase 1 (Sod1) plays pivotal roles in antioxidation via accelerating the conversion of superoxide anion radicals into hydrogen peroxide, thus inhibiting the subsequent radical chain reactions.. While Sod1 deficient cells inevitably undergo death in culture conditions, Sod1-knockout (KO) mice show relatively mild phenotypes and live approximately two years.. We hypothesized that the presence of abundant levels of ascorbic acid (AsA), which is naturally produced in mice, contributes to the elimination of reactive oxygen species (ROS) in Sod1-KO mice.

    Incubation:

    Article Title: Defective biosynthesis of ascorbic acid in Sod1-deficient mice results in lethal damage to lung tissue.
    Article Snippet: Superoxide dismutase 1 (Sod1) plays pivotal roles in antioxidation via accelerating the conversion of superoxide anion radicals into hydrogen peroxide, thus inhibiting the subsequent radical chain reactions.. While Sod1 deficient cells inevitably undergo death in culture conditions, Sod1-knockout (KO) mice show relatively mild phenotypes and live approximately two years.. We hypothesized that the presence of abundant levels of ascorbic acid (AsA), which is naturally produced in mice, contributes to the elimination of reactive oxygen species (ROS) in Sod1-KO mice.

    Article Title: THSG counteracts microglial glycolytic reprogramming and neuronal necroptosis both in vivo and in vitro under conditions of neuroinflammation.
    Article Snippet: .. Cells were then incubated overnight at 4 °C with primary antibodies, either rabbit anti-MAP2 antibody (1:500, #17490-1-AP, Proteintech) or rabbit anti-IBA1 antibody (1:500, #019-19741, Wako). .. After washing with PBS, cells were incubated with secondary antibodies, either Alexa Fluor 594- (#ZF-0516, ZSBG-BIO) or Alexa Fluor 488-conjugated goat anti-rabbit IgG (#ZF-0511, ZSBGBIO) for 2 h at room temperature in the dark.

    Article Title: THSG counteracts microglial glycolytic reprogramming and neuronal necroptosis both in vivo and in vitro under conditions of neuroinflammation
    Article Snippet: .. Cells were then incubated overnight at 4 °C with primary antibodies, either rabbit anti-MAP2 antibody (1:500, #17490-1-AP, Proteintech) or rabbit anti-IBA1 antibody (1:500, #019-19741, Wako). .. After washing with PBS, cells were incubated with secondary antibodies, either Alexa Fluor 594- (#ZF-0516, ZSBG-BIO) or Alexa Fluor 488-conjugated goat anti-rabbit IgG (#ZF-0511, ZSBG-BIO) for 2 h at room temperature in the dark.

    Article Title: Transmission of NLRP3-IL-1β Signals in Cerebral Ischemia and Reperfusion Injury: from Microglia to Adjacent Neuron and Endothelial Cells via IL-1β/IL-1R1/TRAF6.
    Article Snippet: The pyrin domain-containing protein 3 (NLRP3) inflammasome drives the profound cerebral ischemia and reperfusion injury (I/R) and mediates the secretion of IL-1β (interleukin-1β), which exerts a subsequent cascade of inflammatory injury.. The NLRP3-activated-microglial manipulation in adjacent neuronal and endothelial NLRP3 activation has been confirmed in our previous studies.. In the present study, we extended the cognition of how microglia mediated neuronal and endothelial NLRP3IL-1β signaling during cerebral ischemia and reperfusion injury.

    Immunofluorescence:

    Article Title: A Class I HDAC Inhibitor BG45 Alleviates Cognitive Impairment through the CaMKII/ITPKA/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were counted under a microscope by trypan blue, they were placed on poly-L-lysine-coated plates (Solarbio, China). .. The purity of the primary neurons was assessed by immunofluorescence staining with rabbit anti-NeuN antibody (1:200, Abcam, Cambridge, UK, ab177487) and rabbit anti-MAP2 antibody (1:250, Proteintech, Rosemont, IL, USA, 17490-1-AP) after 7 days of culture. .. Cell viability was analyzed by a Cell Counting Kit-8 (Solarbio, China) following the manufacturer’s protocol.

    Staining:

    Article Title: A Class I HDAC Inhibitor BG45 Alleviates Cognitive Impairment through the CaMKII/ITPKA/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were counted under a microscope by trypan blue, they were placed on poly-L-lysine-coated plates (Solarbio, China). .. The purity of the primary neurons was assessed by immunofluorescence staining with rabbit anti-NeuN antibody (1:200, Abcam, Cambridge, UK, ab177487) and rabbit anti-MAP2 antibody (1:250, Proteintech, Rosemont, IL, USA, 17490-1-AP) after 7 days of culture. .. Cell viability was analyzed by a Cell Counting Kit-8 (Solarbio, China) following the manufacturer’s protocol.

    Article Title: RNA Binding Protein Motif 3 Inhibits Oxygen-Glucose Deprivation/Reoxygenation-Induced Apoptosis Through Promoting Stress Granules Formation in PC12 Cells and Rat Primary Cortical Neurons
    Article Snippet: .. With using rabbit anti-MAP2 antibody (catalog no. 17490-1-AP; 1:500 dilution; Proteintech, Wuhan, China), Alexa Fluor488-conjugated goat anti-rabbit antibody (catalog no. ab150077; 1:1000 dilution; Abcam, Cambridge, United Kingdom), and DAPI staining (catalog no. D1306; 300 nM concentration; Invitrogen, Thermo Fisher Scientific, Inc., United States), we confirmed that ≥ 90% living cells were MAP2 positive neurons ( ). .. Images were acquired using the confocal laser scanning microscope (LSM 800, Zeiss, Germany).

    Article Title: Kir6.2 is essential to maintain neurite features by modulating PM20D1-reduced mitochondrial ATP generation
    Article Snippet: Fluorescently labeled sections were visualized with a scanning microscope (Olympus BX51, Japan). .. The primary antibodies used for immunofluorescent staining were as follows: mouse anti-kir6.2 antibody (1:200, Santa Cruz, sc-390104, CA, USA), rabbit anti-GFAP antibody (1:1000, Abcam, ab7206, Cambridge, UK), rabbit anti-MAP2 antibody (1:200, Proteintech, 17490-1-AP, Wuhan, China), rabbit anti-PM20D1 antibody (1:200, Biorbyt, orb555691, Cambridge, UK), and mouse anti-Tau (1:200, Santa Cruz, sc-32274),. .. Mouse brain tissues and cell culture extract lysates were quantified using the QuantiProTM BCA Assay Kit (Sigma, 23235).

    Labeling:

    Article Title: Intraocular vasculature formation precedes extraocular vasculature penetration of mouse eyes.
    Article Snippet: .. The primary antibodies were rat anti-EMCN IgG (MAB2624; Thermo Fisher Scientific Inc., Waltham, MA, USA), rabbit anti-GATA1 IgG (#3535; Cell Signaling Technology [CST], Danvers, MA, USA), goat antiVE-CAD IgG (AF1002) and biotin labeled rabbit anti-PODXL IgG (BAF1556 (both from R & D Systems, Minneapolis, MN, USA), rat antiVEGFR2 IgG (#550549; BD Bioscience, Franklin Lakes, NJ, USA), rabbit anti-MAP2 antibody (Proteintech, Rosemont, Ilm USA), NL557 conjugated mouse anti-β3 tubulin antibody (R&D systems, Minneapolis, MN, USA) and rabbit anti-Hbε antibody (#GTX638775, GeneTex, Irvine, CA, USA). .. The secondary antibodies were Alexa 488 conjugated mouse antialpha smooth muscle actin (184675; Abcam, Cambridge, UK) Alexa 488 labeled anti rat, rabbit, and anti-goat IgG, Alexa 555 labeled anti-rat and rabbit IgG, biotin-labeled rabbit anti-PODXL IgG, and Alexa 488 labeled avidin (all from Thermo Fisher Scientific Inc.).

    Concentration Assay:

    Article Title: RNA Binding Protein Motif 3 Inhibits Oxygen-Glucose Deprivation/Reoxygenation-Induced Apoptosis Through Promoting Stress Granules Formation in PC12 Cells and Rat Primary Cortical Neurons
    Article Snippet: .. With using rabbit anti-MAP2 antibody (catalog no. 17490-1-AP; 1:500 dilution; Proteintech, Wuhan, China), Alexa Fluor488-conjugated goat anti-rabbit antibody (catalog no. ab150077; 1:1000 dilution; Abcam, Cambridge, United Kingdom), and DAPI staining (catalog no. D1306; 300 nM concentration; Invitrogen, Thermo Fisher Scientific, Inc., United States), we confirmed that ≥ 90% living cells were MAP2 positive neurons ( ). .. Images were acquired using the confocal laser scanning microscope (LSM 800, Zeiss, Germany).



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